Immunoway/DAPT/10mg 50mg 100mg/MC0483
市场价：
¥3840.00
美元价：
1920.00
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3392242852
电话号码：
4000-520-616
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info@ebiomall.com
商品介绍
DAPT
- Catalog No.：MC0483
- Data Sheet
- MSDS
- Support
- Description
- References ( 0 )
- Protocol
- CasNo：
- 208255-80-5
- MolecularFormula：
- C23H26F2N2O4
- Purity：
- >98%
- Target：
- γ-secretase
- IC50：
- IC50: 20 nM (Aβ, in HEK 293 cells)[1]
- In Vitro：
- DAPT inhibits Aβ production over 90%, effects only a modest reduction in APPβ in the culture media. Although APPβ is reduced by about 30% by DAPT treatment, this effect is not concentration-dependent and is reversed by the removal of DAPT[1]. CNE-2 cells are treated with increasing concentrations of DAPT (0, 25, 50 and 75 μM), and the γ-secretase-generated Notch 1 fragment Val1744-NICD is decreased after 48 h in a dose-dependent manner (P<0.01). the="" activation="" of="" γ-secretase="" is="" almost="" completely="" inhibited="" by="" dapt="" at="" the="" concentration="" of="" 50="">
- In Vivo：
- DAPT is administered to PDAPP mice (100 mg/kg s.c.) and the levels of DAPT and Aβ are examined in the brain cortex. Peak DAPT levels of 490 ng/g are achieved in the brain 3 h after treatment, and levels greater than 100 ng/g (∼200 nM) are sustained throughout the first 18 h. These brain concentrations of DAPT are in excess of its IC50 for lowering Aβ in neuronal cultures (115 nM), and results in a robust and sustains pharmacodynamic effect[1]. DAPT protects brain against cerebral ischemia by down-regulating the expression of Notch 1 and Nuclear factor kappa B in rats. Western blot analyses also show a significant decrease of Notch 1 and NF-κB expression in DAPT (0.03 mg/kg) group (P<0.05 vs.="" mcao="">
- Fields：
- DAPT(GSI-IX) is an inhibitor of γ-secretase; DAPT causes a reduction in Aβ40 and Aβ42 levels in human primary neuronal cultures (IC50 values are 115 and 200 nM for total Aβ and Aβ42 respectively) and in brain extract, cerebrospinal fluid and plasma.
- Specificity：
- Target:γ-secretase. Fields: DAPT(GSI-IX) is an inhibitor of γ-secretase; DAPT causes a reduction in Aβ40 and Aβ42 levels in human primary neuronal cultures (IC50 values are 115 and 200 nM for total
- Source：
- Rabbit
- Dilution：
- IC50: 20 nM (Aβ, in HEK 293 cells)[1]
- Concentration：
- >98%
- Storage Stability：
- 2 years -20°C Powder, 2 weeks4°C in DMSO,6 months-80°C in DMSO
- Other Name：
- GSI-IX; GSI IX
- MolecularWeight(Da)：
- 432.46
- References：
- [1]. Dovey HF, et al. Functional gamma-secretase inhibitors reduce beta-amyloid peptide levels in brain. J Neurochem. 2001 Jan;76(1):173-81.[2]. Zhou JX, et al. γ-secretase inhibition combined with c
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