Immunoway/53BP1 (phospho Ser6) Polyclonal Antibody/50ug 100ug/YP0710
市场价：
¥3800.00
美元价：
1900.00
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
53BP1 (phospho Ser6) Polyclonal Antibody
- Catalog No.：YP0710
- Applications：WB,IHC-p,IF(paraffin section),ELISA
- Reactivity：Human,Mouse,Rat,Monkey
- Data Sheet
- MSDS
- Support
- Description
- References ( 0 )
- Protocol
- Gene Name：
- TP53BP1
- Protein Name：
- Tumor suppressor p53-binding protein 1
- Human Gene Id：
- 7158
- Human Swiss Prot No：
- Q12888
- Mouse Gene Id：
- 27223
- Mouse Swiss Prot No：
- P70399
- Immunogen：
- The antiserum was produced against synthesized peptide derived from human 53BP1 around the phosphorylation site of Ser6. AA range:1-50
- Specificity：
- Phospho-53BP1 (S6) Polyclonal Antibody detects endogenous levels of 53BP1 protein only when phosphorylated at S6.
- Formulation：
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- Source：
- Rabbit
- Dilution：
- Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/5000. Not yet tested in other applications.
- Purification：
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- Concentration：
- 1 mg/ml
- Storage Stability：
- -20°C/1 year
- Other Name：
- TP53BP1; Tumor suppressor p53-binding protein 1; 53BP1; p53-binding protein 1; p53BP1
- MolecularWeight(Da)：
- 213574
- Observed Band(KD)：
- 213
- Background：
- function:May have a role in checkpoint signaling during mitosis (By similarity). Enhances TP53-mediated transcriptional activation. Plays a role in the response to DNA damage.,PTM:Asymmetrically dimethylated on Arg residues by PRMT1. Methylation is required for DNA binding.,PTM:Phosphorylated at basal level in the absence of DNA damage. Hyper-phosphorylated in an ATM-dependent manner in response to DNA damage induced by ionizing radiation. Hyper-phosphorylated in an ATR-dependent manner in response to DNA damage induced by UV irradiation.,similarity:Contains 2 BRCT domains.,subcellular location:Associated with kinetochores. Both nuclear and cytoplasmic in some cells. Recruited to sites of DNA damage, such as double stand breaks. Methylation of histone H4 at "Lys-20" is required for efficient localization to double strand breaks.,subunit:Interacts with IFI202A (By similarity). Binds to the central domain of TP53/p53. May form homo-oligomers. Interacts with DCLRE1C. Interacts with histone H2AFX and this requires phosphorylation of H2AFX on "Ser-139". Interacts with histone H4 that has been dimethylated at "Lys-20". Has low affinity for histone H4 containing monomethylated "Lys-20". Does not bind histone H4 containing unmethylated or trimethylated "Lys-20". Has low affinity for histone H3 that has been dimethylated on "Lys-79". Has very low affinity for histone H3 that has been monomethylated on "Lys-79" (in vitro). Does not bind unmethylated histone H3.,
- Function：
- function:May have a role in checkpoint signaling during mitosis (By similarity). Enhances TP53-mediated transcriptional activation. Plays a role in the response to DNA damage.,PTM:Asymmetrically dimethylated on Arg residues by PRMT1. Methylation is required for DNA binding.,PTM:Phosphorylated at basal level in the absence of DNA damage. Hyper-phosphorylated in an ATM-dependent manner in response to DNA damage induced by ionizing radiation. Hyper-phosphorylated in an ATR-dependent manner in response to DNA damage induced by UV irradiation.,similarity:Contains 2 BRCT domains.,subcellular location:Associated with kinetochores. Both nuclear and cytoplasmic in some cells. Recruited to sites of DNA damage, such as double stand breaks. Methylation of histone H4 at "Lys-20" is required for efficient localization to double strand breaks.,subunit:Interacts with IFI202A (By similarity). Binds to th
- Subcellular Location：
- condensed chromosome kinetochore,chromosome, telomeric region,nucleus,nucleoplasm,cytoplasm,
- Expression：
- Cerebellum,Cervix,Epithelium,Myeloid leukemia cell,Skeletal muscle,
- June 19-2018
- WESTERN IMMUNOBLOTTING PROTOCOL
- June 19-2018
- IMMUNOHISTOCHEMISTRY-PARAFFIN PROTOCOL
- June 19-2018
- IMMUNOFLUORESCENCE PROTOCOL
- September 08-2020
- FLOW-CYTOMEYRT-PROTOCOL
- July 13-2018
- CELL-BASED-ELISA-PROTOCOL-FOR-ACETYL-PROTEIN
- July 13-2018
- CELL-BASED-ELISA-PROTOCOL-FOR-PHOSPHO-PROTEIN
- July 13-2018
- CELL-BASED-COLORIMETRIC-ELISA-PROTOCOL-FOR-TOTAL-PROTEIN
- July 13-2018
- Antibody-FAQs
- Products Images
- Immunohistochemistry analysis of paraffin-embedded human heart, using 53BP1 (Phospho-Ser6) Antibody. The picture on the right is blocked with the phospho peptide.
- Western blot analysis of lysates from COS7 cells treated with insulin 0.01U/ML 15", using 53BP1 (Phospho-Ser6) Antibody. The lane on the right is blocked with the phospho peptide.
品牌介绍
ImmunowayImmunoWay Biotechnology，我们的使命是帮助世界各地的生命科学研究人员通过更好的产品和服务简化和加速他们的研究。我们专注于开发用于免疫学和细胞学检测的创新和可靠的产品。我们一直是开发高质量ELISA试剂盒，代谢检测试剂盒，抗体和抗体/蛋白质微阵列的先驱，参与癌症，细胞凋亡，细胞生长和信号转导。ImmunoWay Biotechnology，我们的使命是通过更好的产品和服务帮助世界各地的生命科学研究者简化和加速他们的研究。我们专注于开发创新和可靠的产品，用于免疫学和细胞学检测。我们一直是开发涉及癌症，细胞凋亡，细胞生长和信号转导的高质量ELISA试剂盒，代谢测定试剂盒，抗体和抗体/蛋白质微阵列的先驱。
品牌分类
\Elisa试剂盒\
联络我们
