Immunoway/14-3-3 ζ Polyclonal Antibody/50ug 100ug/YT0007
市场价：
¥3200.00
美元价：
1600.00
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
14-3-3 ζ Polyclonal Antibody
- Catalog No.：YT0007
- Applications：WB,IHC-p,IP,IF/ICC,ELISA
- Reactivity：Human,Mouse,Rat
- Data Sheet
- MSDS
- Support
- Description
- References ( 0 )
- Protocol
- Gene Name：
- YWHAZ
- Protein Name：
- 14-3-3 protein zeta/delta
- Human Gene Id：
- 7534
- Human Swiss Prot No：
- P63104
- Mouse Gene Id：
- 22631
- Mouse Swiss Prot No：
- P63101
- Rat Gene Id：
- 25578
- Rat Swiss Prot No：
- P63102
- Immunogen：
- The antiserum was produced against synthesized peptide derived from human 14-3-3 zeta. AA range:24-73
- Specificity：
- 14-3-3 ζ Polyclonal Antibody detects endogenous levels of 14-3-3 ζ protein.
- Formulation：
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- Source：
- Rabbit
- Dilution：
- Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunoprecipitation: 2-5 ug/mg lysate. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/40000. Not yet tested in other applications.
- Purification：
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- Concentration：
- 1 mg/ml
- Storage Stability：
- -20°C/1 year
- Other Name：
- YWHAZ; 14-3-3 protein zeta/delta; Protein kinase C inhibitor protein 1; KCIP-1
- MolecularWeight(Da)：
- 27745
- Observed Band(KD)：
- 28
- Background：
- tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein zeta(YWHAZ)Homo sapiensThis gene product belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 99% identical to the mouse, rat and sheep orthologs. The encoded protein interacts with IRS1 protein, suggesting a role in regulating insulin sensitivity. Several transcript variants that differ in the 5' UTR but that encode the same protein have been identified for this gene. [provided by RefSeq, Oct 2008],
- Function：
- caution:Was originally (PubMed:1577711) thought to have phospholipase A2 activity.,function:Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathway. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner.,PTM:The delta, brain-specific form differs from the zeta form in being phosphorylated (By similarity). Phosphorylation on Ser-184 by MAPK8; promotes dissociation of BAX and translocation of BAX to mitochondria. Phosphorylation on Ser-58 by PKA; disrupts homodimerization and heterodimerization with YHAE and TP53. This phosphorylation appears to be activated by sphingosine. Phosphorylation on Thr-232; inhibits binding of RAF1.,similarity:Belongs to the 14-3-3 family.,subcellular location:Located to
- Subcellular Location：
- extracellular space,nucleus,nucleoplasm,cytoplasm,mitochondrion,cytosol,cell-cell adherens junction,focal adhesion,cytoplasmic vesicle membrane,melanosome,extracellular exosome,
- Expression：
- B-cell lymphoma,Bone marrow
- June 19-2018
- WESTERN IMMUNOBLOTTING PROTOCOL
- June 19-2018
- IMMUNOHISTOCHEMISTRY-PARAFFIN PROTOCOL
- June 19-2018
- IMMUNOFLUORESCENCE PROTOCOL
- September 08-2020
- FLOW-CYTOMEYRT-PROTOCOL
- July 13-2018
- CELL-BASED-ELISA-PROTOCOL-FOR-ACETYL-PROTEIN
- July 13-2018
- CELL-BASED-ELISA-PROTOCOL-FOR-PHOSPHO-PROTEIN
- July 13-2018
- CELL-BASED-COLORIMETRIC-ELISA-PROTOCOL-FOR-TOTAL-PROTEIN
- July 13-2018
- Antibody-FAQs
- Products Images
- Western Blot analysis of various cells using 14-3-3 ζ Polyclonal Antibody diluted at 1：1000
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- Western Blot analysis of COLO205 cells using 14-3-3 ζ Polyclonal Antibody diluted at 1：1000
- Western Blot analysis of 293T HELA using 14-3-3 ζ Polyclonal Antibody diluted at 1:1000. Secondary antibody(catalog#：RS0002) was diluted at 1:20000
- Immunofluorescence analysis of HeLa cells, using 14-3-3 zeta Antibody. The picture on the right is blocked with the synthesized peptide.
- Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using 14-3-3 zeta Antibody. The picture on the right is blocked with the synthesized peptide.
- Western blot analysis of lysates from 293 cells, treated with Forskolin 40nM 30", using 14-3-3 zeta Antibody. The lane on the right is blocked with the synthesized peptide.
品牌介绍
ImmunowayImmunoWay Biotechnology，我们的使命是帮助世界各地的生命科学研究人员通过更好的产品和服务简化和加速他们的研究。我们专注于开发用于免疫学和细胞学检测的创新和可靠的产品。我们一直是开发高质量ELISA试剂盒，代谢检测试剂盒，抗体和抗体/蛋白质微阵列的先驱，参与癌症，细胞凋亡，细胞生长和信号转导。ImmunoWay Biotechnology，我们的使命是通过更好的产品和服务帮助世界各地的生命科学研究者简化和加速他们的研究。我们专注于开发创新和可靠的产品，用于免疫学和细胞学检测。我们一直是开发涉及癌症，细胞凋亡，细胞生长和信号转导的高质量ELISA试剂盒，代谢测定试剂盒，抗体和抗体/蛋白质微阵列的先驱。
品牌分类
\Elisa试剂盒\
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