Immunoway/Homoharringtonine/10mg 50mg 100mg/MC1139
市场价：
¥3400.00
美元价：
1700.00
产品分类：
组织标本
公司分类：
Tissue_samples
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
Homoharringtonine
- Catalog No.：MC1139
- Data Sheet
- MSDS
- Support
- Description
- References ( 0 )
- Protocol
- CasNo：
- 26833-87-4
- MolecularFormula：
- C29H39NO9
- Purity：
- >98%
- Target：
- STAT
- IC50：
- No data
- In Vitro：
- Homoharringtonine inhibits IL-6-induced STAT3 phosphorylation in a dose- and time-dependent manner. Homoharringtonine (HHT) inhibits cells growth, cell viability and colony formation, as well as induced cell apoptosis through mitochondria pathway. The cytotoxicity of Homoharringtonine on human NSCLC cell lines is investigated, A549 (wild type EGFR) and NCI-H1975 (H1975, mutant EGFR with L858R and T790M), Gefitinib is used as a control. By MTT assay, Homoharringtonine has moderate cytotoxicity to A549 with an IC50 of 3.7 μM and H1975 cells are more sensitive to Homoharringtonine with an IC50 of 0.7 μM. Homoharringtonine inhibits the cell proliferation and growth of A549 cells and H1975 cells in a time- and dose-dependent manner through MTT assay. By trypan blue exclusion assay, Homoharringtonine rapidly reduces viable A549 and H1975 cells in a dose- and time-dependent manner. Homoharringtonine significantly inhibits the clonogenic ability of A549 and H1975 cells[1].
- In Vivo：
- Homoharringtonine (10 mg/kg) efficiently represses tumor growth compared to vehicle control or Gefitinib (P<0.05). additionally,="" homoharringtonine="" (hht)="" treatment="" does="" not="" reduce="" the="" mice="" body="" weight,="" which="" suggests="" that="" homoharringtonine="" has="" no="" apparent="" side="" effect.="" all="" the="" mice="" are="" euthanized,="" the="" tumors="" are="" isolated="" and="" imaged="" and="" the="" tumor="" sample="" cells="" are="" harvested="" to="" extract="" protein="" for="" determination="" if="" homoharringtonine="" inhibits="" stat3="" phosphorylation="" via="" western="" blot.="" the="" level="" of="" stat3="" phosphorylation="" and="" mcl1="" from="" homoharringtonine="" treatment="" group="" is="" significantly="" decreased="" compared="" to="" vehicle="" control="" or="" gefitinib="" treatment.="" meanwhile,="" consistant="" with="" the="" results="" in="" the="" above,="" akt1/2/3="" and="" erk1/2="" phosphorylation="" is="" not="" inhibited="" with="" homoharringtonine="" treatment.="" to="" further="" examine="" the="" stat3="" phosphorylation="" in="" the="" xenograft="" tumor="" samples="" with="" different="" treatments,="" the="" tumor="" samples="" are="" frozen="" and="" cutted="" into="" 10 μm="" sections="" for="" fluorescent="" immunohistochemistry.="">
- Fields：
- Homoharringtonine(HHT) is a cytotoxic alkaloid from the evergreen tree.
- Specificity：
- Target:STAT. Fields: Homoharringtonine(HHT) is a cytotoxic alkaloid from the evergreen tree.
- Concentration：
- >98%
- Storage Stability：
- 2 years -20°C Powder, 2 weeks4°C in DMSO,6 months-80°C in DMSO
- Other Name：
- Omacetaxine mepesuccinate; HHT
- MolecularWeight(Da)：
- 545.62
- References：
- [1]. Cao W, et al. Homoharringtonine induces apoptosis and inhibits STAT3 via IL-6/JAK1/STAT3 signal pathway in Gefitinib-resistant lung cancer cells. Sci Rep. 2015 Jul 13;5:8477.
- June 19-2018
- WESTERN IMMUNOBLOTTING PROTOCOL
- June 19-2018
- IMMUNOHISTOCHEMISTRY-PARAFFIN PROTOCOL
- June 19-2018
- IMMUNOFLUORESCENCE PROTOCOL
- June 19-2018
- FLOW CYTOMEYRT PROTOCOL
- July 13-2018
- CELL-BASED-ELISA-PROTOCOL-FOR-ACETYL-PROTEIN
- July 13-2018
- CELL-BASED-ELISA-PROTOCOL-FOR-PHOSPHO-PROTEIN
- July 13-2018
- CELL-BASED-COLORIMETRIC-ELISA-PROTOCOL-FOR-TOTAL-PROTEIN
- July 13-2018
- Antibody-FAQs
品牌介绍
ImmunowayImmunoWay Biotechnology，我们的使命是帮助世界各地的生命科学研究人员通过更好的产品和服务简化和加速他们的研究。我们专注于开发用于免疫学和细胞学检测的创新和可靠的产品。我们一直是开发高质量ELISA试剂盒，代谢检测试剂盒，抗体和抗体/蛋白质微阵列的先驱，参与癌症，细胞凋亡，细胞生长和信号转导。ImmunoWay Biotechnology，我们的使命是通过更好的产品和服务帮助世界各地的生命科学研究者简化和加速他们的研究。我们专注于开发创新和可靠的产品，用于免疫学和细胞学检测。我们一直是开发涉及癌症，细胞凋亡，细胞生长和信号转导的高质量ELISA试剂盒，代谢测定试剂盒，抗体和抗体/蛋白质微阵列的先驱。
品牌分类
\Elisa试剂盒\
联络我们
